Effect of mobile phone radiofrequency electromagnetic radiations on oxidative stress and feeding behaviour in Sprague Dawley (SD) rats

Objectives: Radiofrequency electromagnetic radiation (RF-EMR) from mobile phones is known to produce a stress response because of its effect on hypothalamus. Mobile phones have become an integral part of our lives with increasing usage not only in terms of number of users but also increase in talk time. The present study aimed to study the effect of mobile phone radiofrequency electromagnetic radiations on oxidative stress and feeding behaviour assessment in Sprague Dawley (SD) rats. Materials and Methods: Twelve male SD rats of 10–12 weeks old, weighing 180–220 g, were housed and allowed to acclimatise in a room with 12:12 h light-dark cycle with ad libitum amount of food and reverse osmosis (RO) water before the start of the study. Then, rats were divided into control and RF-EMR exposed groups, and everyday feed intake and body weight were measured. At the end of the study period, blood sample was collected through retro orbital puncture for biochemical investigations. Results: The present study showed significant increase in malondialdehyde and serum corticosterone levels and decrease feeding behaviour in rats exposed to RF-EMR in rats exposed to RF-EMR. Conclusion: This study proves that mobile RF-EMR causes oxidative stress and oxidative damage leading to decreased feeding behaviour in SD rats.

Keratinase mediated fabrication and partial characterization of gold nanoparticles and its antibacterial potential

Keratinase is mainly involved in recycling of keratin waste. Of late, researchers extended its application to nanotechnology. In the present study, we have made an attempt to fabricate and characterize gold nanoparticles using crude keratinase enzyme from Serratia ficaria and also study their biological application, particularly antibacterial activity. The formation of gold nanoparticles (AuNPs) was first verified by UV-Visible Spectroscopy. FTIR spectra confirmed the presence of responsible secondary metabolites for stabilization of nanoparticles. The morphological characteristics and particle size of synthesized nanoparticles were analyzed. The AuNPs showed significant antibacterial activity against Klebsiella pneumonia, Bacillus cereus and Staphylococcus aureus. The highest radical scavenging activity, 60.62% for AuNPs was observed at 500 µg/mL. Results of this study reveals significance of keratinase application in nano-based biological applications.

Influence of Serum Levels of Vitamin D on Insulin Resistance in Patients with Type II Diabetes Mellitus

Background: Vitamin D plays an important role in bone and modulates mineral metabolism and immune function with probable link to several chronic and infectious conditions. In vivo studies have revealed that vitamin D deficiency reduces insulin secretion capacity of the islet beta cells in pancreas. Several studies have shown a correlation between vitamin D levels and insulin resistance, nonetheless, extensive studies showing the relationship between the two are lacking especially among southern Indian population. So the present study was aimed at evaluating the relationship between vitamin D and insulin resistance by using homeostatic model assessment-insulin resistance (HOMA-IR). Materials and methods: In a cross-sectional study, 184 people among which 92 were diabetic and 92 were nondiabetic were recruited at RL Jalappa Hospital, Kolar in the Department of Medicine between May 2018 and April 2019. Fasting serum insulin (I 0), fasting plasma glucose (G0), hemoglobin A1c (HbA1C), renal function test, liver function test (LFT), lipid profile, and vitamin D levels were estimated. IBM SPSS version 22 was used for statistical analysis. Results: The prevalence of vitamin D deficiency in our study was (72) 78.2% among diabetic cases and (59) 64.1% among the nondiabetic controls, with the diabetic cases showing lower levels of vitamin D than the controls, however, it was not statistically significant. There was no significant difference in homeostatic model assessment-beta-cell function (HOMA-B) and HOMA-IR between vitamin D deficient and nondeficient groups among cases and controls. Conclusion: Vitamin D deficiency is prevalent in both type II diabetes mellitus (T2DM) as well as nondiabetic. Furthermore, there is no association between vitamin D deficiency and insulin resistance or beta-cell function.

Study the co-relation between SpO2 and radiological and biochemical markers with the severity and outcome in geriatric Covid-19 patients

Background: Pandemics are natural calamities, which humans have been encountering since time im-memorial. The world has witnessed and endured several pandemics like from Justinian Plague to Spanish Flu. The 21st century has already witnessed three major pandemics like Severe Acute Respiratory Syndrome in 2002, Middle East Respiratory syndrome in 2012 and at present COVID?19. The first case of COVID?19 was reported in Wuhan, Hubein Province in Dec 2019. The first case of COVID?19 was reported in Kerala on 30th January 2020. COVID?19 virus is a single stranded RNA Virus and resembles SARS -2 virus and hence the name SARS -COV 2. There has been a report of around 34 million cases with 1 million deaths. Majority of the deaths occur in the elderly population as they have immunosenescence and multiple comorbidities. Hence a study aiming at analysis of correlation between SpO2, radiological and biochemical markers is essential in management of COVID 19 in elderly. Aims & Objectives: To study the correlation between SpO2 and radiological features in X ray and CT Chest and biochemical markers with the severity and outcome in geriatric patients. Study Period: April 2020 to September 2020, Duration: 6 months. Study Centre: Chengalpattu Medical College, Tamilnadu. Inclusion Criteria: RT PCR positive Covid 19 patients more than 60 years of age. Exclusion Criteria: Covid 19 patients less than 60 years, RT PCR Negative patients. Materials and Methods: Retrospective analysis of records of 300 Covid 19 patients, aged more than 60 years hospitalized in Chengalpattu Medical College during April to September 2020 was done and biochemical parameters mentioned below were corelated with features in CXR and CT Chest RTPCR positive spo2 and analysis with severity of disease and outcome was done. Results and Discussion: On retrospective analysis of records of elderly Covid 19 patients, it was observed that the elderly could be graded as mild, moderate and severe based on clinical criteria. Radiological parameters co-relating in the same group classified as mild, moderate and severe could be derived and could be used for assessment of patients. Similarly, it was observed that the biochemical parameters of D? Dimer, C reactive protein, Neutrophil Lymphocyte Ratio correlated most significantly in that order with the clinical severity of disease and hence could be used as useful tools for assessment of severity of patients and the ranges corelating withseverity could be derived. Conclusion: Analyzing SpO2, radiological features, biochemical parameters individually and in combination with clinical severity and outcome in elderly patients helps in deriving biochemical and radiological parameters to predict severity which in turn helps in a holistic approach and right management at the right time preventing or reducing mortality due to Covid 19 in the elderly.

Impact of the novel coronavirus disease and lockdown on the packed red blood cells inventory management: An experience from a tertiary care oncology center in Western India

ABSTRACT Introduction The novel coronavirus disease has disrupted blood transfusion services worldwide. Despite blood transfusion services taking several precautionary measures to minimize the risks of COVID-19 during blood donations, donors became anxious regarding the risk of the COVID-19 infection during the donation and the blood transfusion services was facing the inevitable hazard of blood shortage. Methods The study was conducted at a tertiary care oncology hospital-based blood transfusion services and included analysis of blood donations, packed red blood cell units requirements, and packed red blood cell inventory in the pre lockdown and lockdown phase. New COVID-19 standard operating procedures with enhanced safety guidelines and donor confidence-building measures were implemented at the blood transfusion services. Results The total number of average monthly blood donations decreased in lockdown but the decrease was not statistically significant (238.5 vs. 197.8, P = 0.391). The requisitions for the packed red blood cell cross-matches (722.5 vs. 329.0, P = 0.001) and the packed red blood cell utilization (176.5 vs. 103.3, P = 0.028) for the hospital patients also decreased significantly due to the lockdown. In the lockdown phase, an expressive number of packed red blood cell units were outdated due to the unprecedented fall in the number of patients. In the post-lockdown phase, the packed red blood cell inventory was optimized with decreased outdating via a comprehensive approach. A special emphasis was given to the in-house donations. A second partial lockdown also decreased the blood donations. Conclusion Confidence-building in blood donors and the resolution of logistical issues were crucial for the efficient packed red blood cell inventory management in the lockdown. Implementation of COVID-19 preventive measures helped in the blood donor and blood transfusion services staff safety.

Diagnosing Herpetic Genital Ulcers in a Resource-Limited Setting- What Works?

Aims: Globally, viral agents, especially herpes simplex virus (HSV), have overtaken the bacterial causes of genital ulcers. Very few laboratories in India, perform culture techniques and polymerase chain reaction (PCR) for diagnosis of genital ulcers. This study aimed to establish the utility of existing tests, which are cheaper and need less technical expertise, when compared to newer tests such as PCR.Study Design: This cross sectional study was carried out to determine the aetiology of genital ulcers, with emphasis on diagnosis of herpetic ulcers, using newer and more accurate methods of diagnosis and evaluating their performance by comparing against viral culture as gold standard test.Place and Duration of the Study: The study was carried out over a period of one year in the Apex Regional Sexually Transmitted Diseases (STD) Centre at Safdarjung Hospital, New Delhi and the Department of Microbiology, AIIMS, New Delhi.Methodology: Fifty three patients with genital ulcers were included in the study. Specimens from ulcers were taken for various tests, including Giemsa stain, ELISA for HSV-1 & 2, PCR and Viral culture for HSV.Results: HSV was identified in 31 of 53 cases (58.5%), including 03 cases of HSV-1, and 28 cases of HSV-2. Sensitivity and specificity of PCR was 90.0% and 84.85%, respectively. Viral culture positivity was 37.7%.Conclusion: Genital herpes is associated with an increased risk of Human Immunodeficiency Virus (HIV) acquisition, and clinical manifestations are diverse; hence a presumptive diagnosis should be confirmed by reliable laboratory tests. Nucleic acid amplification tests (NAAT) are the most sensitive methods for direct detection of HSV. The extensive validation of these tests allows for their application in routine laboratory settings with consistency and greater diagnostic accuracy. When standardised and used, PCR is a highly reproducible, rapid and labour efficient method for HSV detection.

Histological and Immunohistochemical Studies of Furstenberg’s Rosette in Sheep and Goat

A total of twelve healthy adult Madras Red ewe and Boer local she-goat teats were histologically and immunohistochemically examined. In between the teat canal and teat cistern Furstenberg’s rosette was appeared as modified zone of mucosa thrown into primary and secondary folds. The mucosal folds were lined by a bilayered cuboidal epithelium. Subepithelial area of rosette was composed of numerous lymphocytes, polymorphonuclear leukocytes, macrophages and plasma cells. Mucosa below the Furstenberg’s rosette was lined by stratified squamous keratinized epithelium. Immunohistochemical staining of rosette by CD3 antibody revealed the localization of intraepithelial ‘T’ lymphocytes in the mucosal folds. ‘T’ lymphocytes were also localized in the lamina propria region

Histological Study of Cerebrum, Cerebellum in Anencephalic & Non-Anencephalic Foetuses.

Anencephaly is one of the most common birth defects. This was a hospital-based case finding study that covered 60 patients with anencephaly & their respective mothers, conducted in hospitals in Bangalore Medical College & Research Institute from 2014 to 2017.METHODSThe study included 60 anencephalic foetuses (23 males & 37 females) of 20-30 weeks & 20 non-anencephalic foetuses (9 males & 11 females). Maternal history was analysed using a questionnaire which includes age factor, environmental factors, medications, family history, consanguineous marriage and febrile illness during pregnancy. The foetuses were examined for external abnormalities & dissected. Dissected foetal cerebrum & cerebellum tissues were processed & stained with H&E using tissue processor.RESULTSIn 100% of cases, all layers of cerebrum & cerebellum of non-anencephalic foetuses were normal. In anencephalic foetuses, cerebrum in 75% cases showed primitive brain cells, astrocytes & glial cells, instead of 5 layers which was described in checklist & in 25 % cases angiomatous masses were seen but primitive brain cells & astrocytes were absent. 91.7% cases of anencephalic foetal cerebellum had 5 cell stages which were the same as non-anencephalic foetuses & 8.3% cases had all the layers but ill formed granular layer. Most of the organs were normally developed. Associated anomalies were also noticed in 70% of cases.CONCLUSIONSThe study emphasizes the complexity of the aetiology behind anencephaly, variability of its presentation & yet unsatisfactory awareness among mothers about folic acid & its beneficial role in preventing anencephaly.

Distalization of the entire maxillary arch with mini-implants in the posterior palatal alveolus- A case report / Distalização de todo o arco maxilar com mini-implantes na região alveolar posterior palatina - Relato de caso

A 22 year old male patient reported to the hospital with a chief complaint of forwardly placed teeth. On examination patient had Angle's Class I malocclusion and proclination of the anterior teeth. There were signs of frictional keratosis on the buccal mucosa. Treatment plan was to extract the third molars alone and distalize the entire maxillary arch with palatal mini-implants. 0.022 MBT brackets were bonded on the buccal aspect. 0.019" x 0.025" stainless steel wire was placed sequentially. Mini-implants were placed on the posterior alveolus on the palatal surface of maxilla. Retractive force was applied from an attachment bonded on the palatal aspect of the maxillary canine. Patient was reviewed periodically. Comparison of pre-treatment and post-treatment results revealed that the entire maxillary arch intruded and translated distally with a counter-clockwise rotation of the mandible with reduction in LAFH. There was a mild reduction in inter-canine with marginal expansion in the premolar and molar region. An improvement in facial profile was noted with no sign of root resorption. Thus, the posterior alveolus may be considered as a new and appropriate site for placement of mini-implant to bring about distal movement of the entire maxillary dentition (AU)

Um Paciente do sexo masculino, 22 anos, foi encaminhado ao hospital com queixa principal de dentes posicionados para a frente. Ao exame clínico o paciente apresentava má oclusão de Classe I de Angle e inclinação vestibular dos dentes anteriores. Havia sinais de queratose friccional na mucosa bucal. O plano de tratamento foi extrair os terceiros molares e distalizar todo o arco maxilar com mini-implantes por palatino. Bráquetes MBT 0,022 foram colados por vestibular. Fio de aço inoxidável 0,019 "x 0,025" foi colocado seqüencialmente. Mini-implantes foram instalados na região alveolar posterior da superfície palatina da maxila. A força de retração foi aplicada a partir de acessórios colados nas faces palatinas dos caninos superiores. O paciente foi reavaliado periodicamente. Os resultados da comparação pré-tratamento e pós-tratamento revelaram que todo o arco maxilar intruiu e transladou distalmente com rotação da mandíbula no sentido anti-horário com redução da AFAI. Houve uma ligeira redução na distância intercanina com expansão marginal nas regiões de pré-molar e molar. Foi percebida melhora no perfil facial sem sinal de reabsorção radicular. Assim, a região alveolar posterior pode ser considerada como um novo e apropriado local para instalação de mini-implante para promover movimento distal de toda a dentição maxilar. (AU)

Rapid burst of ethylene evolution by premature seed: A warning sign for the onset of spongy tissue disorder in Alphonso mango fruit?

Moisture stress induced in premature seeds due to the breakdown of funiculus in Alphonso mango led to the burst ofethylene evolution, which in turn caused a sudden increase of polyphenol oxidase activity in the pulp, resulting in thedevelopment of a black spot near the seed base. Reduced levels of very long chain fatty acids in 70% mature seeds withblack spots were associated with a sudden increase of cytokinins followed by a rapid rise of starch-metabolizing enzymesculminating in the onset of pre-germination events. Concurrently, an overproduction of p-OH benzoic acid inhibitedamylase and polygalacturonase enzymes and led to partial degradation of the stored starch and pectin in the pulp. A paralleldrop in climacteric ethylene production by the pulp led to incomplete ripening coupled with changes in composition, textureand aroma of the pulp, characteristic of spongy tissue. The results have provided strong experimental evidence to supportthe fact that increased competition for resources among developing fruits for the synthesis of seed fat plays a critical role inspongy tissue formation in Alphonso mango. The major highlight of the study is that rapid ethylene evolution by prematureseed is an early warning sign for the initiation of spongy tissue formation in Alphonso mango.

A prospective study on the role of umbilical artery doppler velocimetry in the perinatal outcome of growth restricted fetuses

Background: To compare pregnancy outcomes in growth-restricted fetuses retaining normal umbilical artery doppler flow and the outcomes of pregnancies with end-diastolic velocity diminished or severely reduced/absent.Methods: In a prospective observational study, one hundred pregnant women with growth-restricted fetuses were followed with doppler velocimetry of the umbilical artery between weeks 28 and 41 of pregnancy. Outcomes were compared for the normal doppler group (55%) (Group 1), the low-end diastolic flow group (32%) (Group 2), and the group with severely reduced or absent end-diastolic velocity waveforms (13%) (Group 3).Results: Fetuses with abnormal umbilical flow velocimetry had higher incidence of oligohydramnios (82.2%). The average birth weight and gestational age at delivery were lower in the abnormal doppler group. Significantly more women with severe reduction/AEDV, 31/45 (68.8%), underwent caesarean section, with 20 of them (44.4 %) for fetal distress, compared with 17/55 (30.9%) women in the normal doppler group, with 9 of them (16.4%) for fetal distress. Also, fetuses with abnormal umbilical artery velocimetry had early delivery at less than 36 weeks of gestation (38.2% versus 65.85%), increased NICU admission (32.7% versus 80%), need for PPV (5.5% versus 40%), low Apgar score (9.1 % versus 50.2%) than those with normal doppler. All the three stillbirths in the study population were in the abnormal doppler group.Conclusions: Umbilical artery doppler velocimetry helps in differentiating fetus with pathological growth restriction at risk for perinatal complications from small and healthy fetuses.

Effectiveness of healthy eating and active lifestyle intervention program on physiological parameters among children with obesity

Background: The aim of the study was to investigate the effectiveness of Healthy Eating and Active Lifestyle Intervention Program (HEALIP) on physiological parameters among children with obesity.Methods: True experimental research design was adopted with 200 children between the age group of 12-18 years who met the inclusion criteria from four different schools in Chennai, Tamil Nadu, India. Samples were allocated into experimental group (n=100) and control group (n=100) by random sampling technique. Children were screened for weight, height, body mass index, and blood pressure were measured before as well as after the intervention. Healthy eating and active lifestyle intervention program were administered for the experimental group for 16 weeks, whereas the control group received routine practices. Post-test was conducted at the end of the 16th week for the both groups. Data were analysed using SPSS.Results: There was a difference in the pre-test systolic blood pressure (128.4'1.3) and post-test (118.6'0.7) and in the pre-test diastolic blood pressure (78.8'0.6) and post-test (72.8'0.6) mean value in the experimental group and found statistically significant at the level of p <0.001. It was also observed the significant difference between the experimental and control group.Conclusions: The finding of the study concluded that healthy eating and active lifestyle intervention program has proved the beneficial effect in maintaining the physiological parameters. Hence, it was concluded that there is a need to educate the children about the obesity and its complications. The study findings ensure that periodic monitoring of the basic physiological parameters among obese children will prevent the furthermore complications.

Neutrophil and Platelet to Lymphocyte Ratio in Prevailing the Oxidative Stress and its Relation with the Endothelial Dysfunction in Preeclampsia

Background: Preeclampsia (PE) is a pregnancy specific,hypertensive disorder. It affects 2-8% pregnancies.Oxidative stress and systemic inflammation areproposed to contribute significantly to the preeclampsiapathophysiology. The present study, aim is to determineand compare the markers of oxidative stress, endothelialdysfunction, systemic inflammatory markersNeutrophil/Lymphocyte Ratio (NLR) and Platelet/Lymphocyte Ratio (PLR) in preeclampsia andgestational age matched healthy controls. Material andMethods: This study was conducted in the Departmentof Biochemistry and Department of Obstetrics andGynecology, Sri Devaraj Urs Medical College, Kolar,Karnataka. The study included 98 preeclamptic womenand 98 normotensive pregnant women. Five ml venousblood was collected from all the study subjects. Bloodsample in EDTA vials was used for the complete bloodcount. NLR and PLR were calculated. Plasma was usedfor Ferric Reducing Ability of Plasma (FRAP) assay.Serum was used for the estimation of Malondialdehyde(MDA), nitric oxide, blood sugar, renal parameters andliver enzymes i.e., Aspartate Transaminase (AST),Alanine Transaminase (ALT), Lactate Dehydrogenase(LDH) and magnesium. Corresponding urine sampleswere collected for urinary protein analysis by dipstickmethod. Fetal outcome was recorded. Results:Gestational age was significantly low in preeclampticwomen as compared to those of controls. Bloodpressure (Systolic and diastolic), mean arterial pressure,body mass index, pulse rate, serum creatinine, uric acid,AST, ALT, LDH, MDA and NLR were increasedsignificantly in preeclamptic women as compared tothose of controls. In subgroup analysis, NLR wasincreased significantly in severe preeclamptics ascompared to mild preeclamptics. Serum Nitric Oxide(NO) and FRAP levels were decreased significantly inpreeclamptic women as compared to those of controls.Significantly decreased birth weight was observed inbabies born to preeclamptic mothers compared withcontrols. Conclusion: The present study resultsconclude that increased oxidative stress in termsincreased MDA, decreased NO and reduced antioxidantstatus (FRAP) in preeclamptic women, results inadverse perinatal outcome. In addition, maternal NLRcould be considered as a marker for severity ofpreeclampsia

Clonal clusters and virulence factors of methicillin-resistant Staphylococcus Aureus: Evidence for community-acquired methicillin-resistant Staphylococcus Aureus infiltration into hospital settings in Chennai, South India

Background and Objective: Staphylococcus aureus is one of the major pathogens of nosocomial infections as wells as community-acquired (CA) infections worldwide. So far, large-scale comprehensive molecular and epidemiological characterisation of S. aureus from very diverse settings has not been carried out in India. The objective of this study is to evaluate the molecular, epidemiological and virulence characteristics of S. aureus in both community and hospital settings in Chennai, southern India. Methods: S. aureus isolates were obtained from four different groups (a) healthy individuals from closed community settings, (b) inpatients from hospitals, (c) outpatients from hospitals, representing isolates of hospital–community interface and (d) HIV-infected patients to define isolates associated with the immunocompromised. Antibiotic susceptibility testing, multiplex polymerase chain reactions for detection of virulence and resistance determinants, molecular typing including Staphylococcal cassette chromosome mec (SCCmec) and agr typing, were carried out. Sequencing-based typing was done using spa and multilocus sequence typing (MLST) methods. Clonal complexes (CC) of hospital and CA methicillin-resistant S. aureus (MRSA) were identified and compared for virulence and resistance. Results and Conclusion: A total of 769 isolates of S. aureus isolates were studied. The prevalence of MRSA was found to be 7.17%, 81.67%, 58.33% and 22.85% for groups a, b, c and d, respectively. Of the four SCCmec types (I, III, IV and V) detected, SCCmec V was found to be predominant. Panton-Valentine leucocidin toxin genes were detected among MRSA isolates harbouring SCCmec IV and V. A total of 78 spa types were detected, t657 being the most prevalent. 13 MLST types belonging to 9 CC were detected. CC1 (ST-772, ST-1) and CC8 (ST238, ST368 and ST1208) were found to be predominant among MRSA. CA-MRSA isolates with SCCmec IV and V were isolated from all study groups including hospitalised patients and were found to be similar by molecular tools. This shows that CA MRSA has probably infiltrated into the hospital settings.

Ultrasound Anatomy of the Thyroid Gland in Dogs

Ultrasonography is a method of choice for imaging visceral organs in animals. The aim of the study was to determine the ultrasonographic characteristics of the thyroid gland in healthy dogs and to correlate the relationships of thyroid gland size and volume with body weight. A total of 24 apparently healthy dogs of different breeds were grouped into four groups (each with 6 animals) based on their body weight viz., less than 7 Kgs, 7-15 kgs, 15-30 kgs and more than 30 kgs. Each thyroid lobe was ultrasonographically observed in both transverse and longitudinal planes. The maximal length, width and height of each lobe and thyroid volume were measured. The data were analyzed statistically. In all the groups, thyroid lobes were round to oval in transverse images and fusiform or elliptical in longitudinal images with the smooth capsule. The parenchyma of the thyroid lobes had a homogenous echogenic pattern. There was no significant difference observed between the mean height, length, width and volume of both the lobes of the thyroid gland with body weight among the four groups at 5% level of significance (p< 0.05)

Role of FNAC in Chronic Childhood Lymphadenopathy with Suspected Tuberculosis

Introduction: Tuberculous lymphadenitis is one of the common cause of Paediatric lymphadenopathy in India. This study was aimed to assess the usefulness of Fine needle aspiration cytology of lymph nodes as a diagnostic tool in suspected Tuberculosis and to observe the cyto morphological changes for better understanding of immune response. Material and methods: This was a cross sectional observational study conducted between the year 2016 to 2018 at Melmaruvathur tertiary care hospital. Children up to 14 years with suspected Tuberculosis symptoms and chronic lymphadenopathy irrespective of the sizes of nodes were subjected to Fine needle aspiration cytological study. Cyto smears morphology were observed and analysed. Results: A total of 81 cases were studied and the predominant age group affected were between five to ten years (n=46; 56.8%). 42 patients were female (51.9%) and 39 were male (48. 1%).Most of them had cervical nodes and 44% of nodes were less than 1 cm size. Out of 81 cases, 32 (39.5%) were diagnosed as nonspecific adenitis and 46 cases (56.8%) as granulomatous lymphadenitis. Among the size of the nodes, 57% of small nodes, 65% of medium sized nodes and 100% of large nodes showed granulomatous adenitis. Among sexes, female preponderance (63.1%) for granulomatous adenitis and male predominance (62.5%) for nonspecific adenitis were observed. Conclusion: Fine needle aspiration cytological study of paediatric lymphadenopathy is a useful diagnostic indicator in suspected Tuberculosis even for small size nodes and can be recommended if clinically warranted.

M-mode Echocardiographic Studies in Indigenous Dog Breeds of Tamil Nadu

M-mode echocardiography is a non- invasive tool for the quantitative study of heart. Rajapalayam is a guard dog and Chippiparai is a sight hound breed which both originated from the southern districts of Tamil Nadu, India. Diagnosis of cardiac diseases in our indigenous breeds has been challenging due to less or no work done regarding cardiac anatomy and physiology in native breeds. Hence, this study was undertaken to establish basic reference data in these indigenous dog breeds which will help in diagnosing cardiac diseases. Various normal cardiac parameters were documented between young and adult age groups. The results indicated that a high significant difference (P<0.01) was observed in LVDd and LVDs and only a significant difference (P<0.05) in AO and RVD values between the age groups in Rajapalayam dogs,whereas, in Chippparai dogs, a significant difference was observed in CO (P<0.05) and in LVPWs, IVSd, IVSd (P<0.0) between the young and adult age groups

A three-dimensional finite element analysis to evaluate stress distribution tooth in tooth implant-supported prosthesis with variations in non-rigid connector design and location

The physiologically mobile natural tooth and rigidly fixed dental implant causes different distribution of stress when connected in prosthesis and nonrigid connector compensates this. Understanding of biomechanical behavior is necessary for an adequate choice and construction of this type of rehabilitation. However, there has been insufficient research focusing on different location and type of the nonrigid connector related with the prognosis of both implant and the tooth. Aim of the Study: The purpose of this finite element (FE) analysis was to evaluate the stress distribution around bone, implant, and tooth in tooth implant fixed prosthesis under static load with variations in design and location of nonrigid connectors under simulated functional loads. Materials and Method: Three, 3-dimensional FE models connecting tooth and implant were constructed with different location and type of nonrigid connector. Simulated occlusal load was applied on the restorations and stresses developed in the supporting structures were monitored. Results: The highest stresses were found around the implant in model with nonrigid connector placed between the tooth and implant and model with modified nonrigid connector. On the other hand, less stress was noted around the implant where nonrigid connector was placed between the implant and pontic. Conclusion: It is advisable to place the nonrigid connector between the implant and the pontic to protect the implant from torque effects in a tooth implant fixed prosthesis.

Achalasia Cardia In Indian Children– Experience From A Tertiary Care Institute In Chennai, India

Introduction:Achalasia cardia a rare oesophageal motility disorder is not uncommon in children. Aim and objectives:To describe the clinical profile of achalasia from a single pediatric tertiary care centre. Materials and Methods: Retrospective analysis of case records over a period of 10 years from March 2008 to March 2018 at institute of child health and hospital for children, Chennai, India. Results: 26 children had achalasia and 11 (42.3%) were males. The mean age was 42.08±36.33 months and 19 (73.07%) were less than five years at diagnosis. Vomiting was the most common symptom (88.4%) followed by failure to thrive (80.77%). Six had features of Allgrove syndrome. Heller's cardiomyotomy with or without fundoplication was done in 24 (92.3%) and pneumatic dilatation in two. Conclusion: Achalasia cardia is not uncommon and should be considered in children presenting with vomiting, dysphagia and failure to thrive. Surgery remains to be safe and effective.

Labor Admission Test And Neonatal Outcome

Purpose: Purpose of the study is to study the Mode of Delivery and Perinatal outcome in relation to the admission test in the study group . Method: All women in latent phase of labor were subjected to admission test with CTG machine and tracing recorded. Any complication arising during labor and the perinatal out come were recorded. Results: Incidence of fetal distress, need for operative delivery, low apgar scores, low birth weight, admission to NICU and perinatal deaths are significantly more in non reactive admission test group. Conclusion: Admission test is economical, non invasive, and readily available screening test. Non reactive patterns predicts well the incidence of perinatal complications and Neonatal mortality.